Putih Telur Sebagai Protein Blocking Pada Pengecatan Estrogen Receptor Metode Imunohistokimia

Abstract

albumen is an animal protein with a high protein content, so it might can be used as a blocking agent that can inhibit non-specific protein found in tissues. One of breast cancer diagnostic method is by IHC ER (Estrogen Receptor) staining. ER is a protein produced by tissue that produce gene mutations in DNA that induring cells proliferation. The objective of the research was to find out the results of IHC ER staining using albumen from organic chicken egg and albumen from duck egg with concentration of 1%; 1,5%; 2% and 2,5% and normal serum as control. The sample was 1 block of IHC ER +3 breast cancer paraffin. Indirect IHC staining using Strep (Avidin) Biotin Complex method. IHC ER staining using normal serum as control resulted +3. The best concentration on IHC staining using albumen from organic chicken egg is at concentration 2,5%, albumen from duck egg concentration 2% and 2,5% with the result of +3. There was no significant difference between normal serum and albumen from organic chicken egg with concentration of 2,5%, albumen from duck egg with concentration of 2% and 2,5%. The conclusion is a normal serum can be replaced with 2,5% albumen from organic chicken egg , 2% and 2,5% concentration of albumen from duck egg.